Cilt:29 Sayı:01 (2020)

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https://dspace.ankara.edu.tr/handle/20.500.12575/76572

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Antımutagenıc and antıoxıdant actıvıtıes of teucrıum multıcaule and ıts cytotoxıc effect on murıne lr7 cell
(Ankara Üniversitesi Fen Fakültesi, 2020) Pehlivan, Mustafa; Other; Other
In present study, DNA protection, antioxidant and cytotoxic effects of Teucrium multicaule Montbret Et Aucher Ex Bentham methanol extract (TME) was investigated. Cytotoxic effects and antioxidant activities of TME was determined by MTT and DPPH scavenging and DNA protection methods. As a result, high dose of TME exhibited a cytotoxic effect on murine L cells. Although, TME also showed a strong antimutagenic activity in DNA protection test system, it exhibited modest activity in DPPH test system. Consequently, it may be used as natural agent for antioxidant and antimutagenic properties.
Bıofılm formatıon and control of facultatıve thermophıle brevıbacıllus Agrı D505B
(Ankara Üniversitesi Fen Fakültesi, 2020) Cihan, Arzu Çöleri; Biyoloji; Fen Fakültesi
Brevibacillus agri D505b is an aerobic, non-pathogenic, endospore-forming facultative thermophilic bacillus. Six abiotic surfaces (stainless steel, glass, polyvinyl chloride, polypropylene, polystyrene, and polycarbonate) were compared with viable cell enumerations. According to results, D505b cells could able to attach all these surfaces. Stainless steel (6.10 log CFU/cm2) was found to be the most effective surface for biofilm formation. Polycarbonate (6.03 log CFU/cm2) was found as the second best surface. Furthermore, the D505b biofilm was treated with 15 different sanitation agents and trichloroacetic acid (TCA) was determined to be the most effective one (80.3% removal). Our results showed that the strong biofilm producer B. agri D505b was very resistant to various sanitation agents. However, TCA significantly inhibited biofilm formation for the isolate.
Classıfıcatıon of turkısh honeys from aydın-karacasu-dıkmen vıllage based on melıssopalynologıcal parameters
(Ankara Üniversitesi Fen Fakültesi, 2020) Çelemli, Ömür Gençay; Other; Other
The classification of Aydın-Karacasu-Dikmen honeys was practiced based on melissopalynological parameters. A total of 65 honey samples from Aydın-Karacasu-Dikmen village located in Aegean Region of Turkey were collected during the 2018-2019 harvesting season. According to the melissopalynological results, 54 samples were determined as nectar (blossom), seven as honeydew honey and four as mix of nectar and honeydew honey (blend honey). In all the honey samples Thymus spp. pollens were observed. Also sensory analysis were done for the investigated honey samples. As a result, owing to Thymus spp. pollen contents in all the samples the aroma and the odour of Thymus were detected by sensory analyses. The honey types of the region were determined according to the botanical sources exhibited by the research.
Comparatıve bıometrıc and morphologıcal analysıs of subgenus terrıcola (rodentıa: mıcrotus) ın eastern black sea regıon from Turkey
(Ankara Üniversitesi Fen Fakültesi, 2020) Çolak, Ercüment; Biyoloji; Fen Fakültesi
In the scope of current study, 41 specimens of the subgenus Terricola collected from nine different localities in Trabzon, Rize and Artvin provinces in Eastern Black Sea Region were subjected to detailed morphological and comparative biometric analyses. Based on those analyses; presence of two species, Microtus (Terricola) subterraneus and Microtus (Terricola) majori were determined in the region. Evaluation of the 12 specimens belonging to M. (T.) subterraneus showed that there is no intrapopulational variation within this species. Two different populations of M. (T.) majori were determined in the region studied, based on morphological differentiations in enamel cusp patterns of 29 specimens. Additionally, as a result of the evaluations made, it was determined that both species could be found in the similar habitats throughout study area and therefore that these two species can be coexist as sympatric within the same geographic area.
Determınatıon of fusarıum specıes in carnatıon greenhouses in Antalya, Turkey
(Ankara Üniversitesi Fen Fakültesi, 2020) Atakan, Aydın; Other; Other
In this study, revealing of the identification and pathogenicity of Fusarium species isolated in carnation greenhouses of Antalya, Turkey were aimed. As a result of isolations, fungi included in Fusarium genus were identified using macroscopic and microscopic techniques. Pathogenicity of identified species were determined using Turbo carnation cutivar. As a result of the diagnostic studies, species belonging to the genus Fusarium were determined as F. acutatum, F. avenaceum, F. chlamydosporum, F. equiseti, F. oxysporum, F. poae, F. proliferatum, F. sambucinum, F. solani, F. tricinctum and F. verticillioides. F. oxysporum is the most commonly isolated species According to the pathogenicity test in Turbo carnation variety, the disease severity of Fusarium species were changed between 60-88 % and determined that F. solani had the highest pathogenicity rates among the others. Consequently, a total of 11 Fusarium species have been identified and has been demonstrated that have potential to cause problem in carnation cultivation in greenhouses in Antalya, Turkey.
Effects of β-estradıol on dna methylatıon changes and genomıc stabılıty ın trıtıcum aestıvum l. exposed salt
(Ankara Üniversitesi Fen Fakültesi, 2020) Aydın, Murat; Other; Other
Salinity stress is considered one of the most important agricultural problems because it causes yield loss. Although it is well known that salinity damages to DNA and results in DNA methylation changes in plants, there is no report investigating the effect of mammalian hormones in plants growing under salinity stress. Therefore, the present study wasa imed at investigating DNA damage levels, DNA stability and DNA methylation changes in Triticum aestivum subjected to salinity stress and determine whether β-estradiol has any effect on these changes. RAPD (Randomly Amplified Polymorphic DNA) and CRED-RA (Coupled Restriction Enzyme Digestion-Random Amplification) were used to define the DNA damage levels and changes in the pattern of DNA methylation. The results showed that salinity stress led to an increase in RAPDs profile changes (DNA damage), a reduction in genomic template stability (GTS) and DNA hypermethylation. The effects caused by salinity stress were decreased after treatment with different concentrations of β-estradiol. The results of this study clearly show that β-estradiol could be used effectively to protect wheat seedlings from the destructive effects of salinity stress, and demethylation positively contributed to salinity stress tolerance.
Ennomınae, geometrınae and larentıınae (lepıdoptera: geometrıdae) fauna ın Gazıantep provınce, Turkey
(Ankara Üniversitesi Fen Fakültesi, 2020) Kütük, Murat; Other; Other
This study was based on Geometridae species collected from Gaziantep province in spring and summer of 2009, 2010 and 2011. During the study, samples were collected from research area using by Robinson type light trap, portable light trap and insect net. In the study, 14 species belonging to 14 genera from 3 subfamilies have been determined. In addition, adult figures all species were given.
Flora of Çaltepe and Çeletepe (Bolu)
(Ankara Üniversitesi Fen Fakültesi, 2020) AYDIN, Çelik; Eker, İsmail; Other; Other
This study was carried out to reveal the vascular plant diversity of Çaltepe and Çeletepe. During 2015−2018, 2340 plant specimens were collected from the research area and 363 genera and 767 taxa belonging to 81 families were determined. Of all the collected taxa, 66 are endemic and endemism rate is %8.60. The IUCN threat categories of endemic and rare plants at global level are as follows: 1 taxon in “CR” category, as well as 4 taxa “EN”, 4 taxa “VU”, 9 taxa “NT”, and 45 taxa “LC”. Also, 3 rare taxa are found in the “VU” category, as well as 1 rare taxon in the “DD” category at regional level. 56 taxa are new records for the province of Bolu. The largest families in the study area are as follows: Asteraceae 97 taxa (%12.65), Fabaceae 62 taxa (%8.08), Lamiaceae 51 taxa (%6.65), Rosaceae 44 taxa (%5.74), Poaceae 37 taxa (%4.82), Brassicaceae 35 taxa (%4.56), Caryophyllaceae 32 taxa (%4.17), Apiaceae 28 taxa (%3.65), Boraginaceae 27 taxa (%3.52) and Orchidaceae 24 taxa (%3.13). The distribution of taxa into phytogeographic regions are as follows: 234 taxa (%30.50) Euro-Siberian, 64 taxa (%8.34) Mediterranean, 46 taxa (%5.99) Irano-Turanian, and 423 taxa (%55.15) multiregional and/or unknown. Geranium and Silene (12 taxa) are the most common genera in the research area. These genera are followed by Salvia (11 taxa), Trifolium, Veronica and Campanula (each with 10 taxa), Ranunculus, Euphorbia and Vicia (each with 8 taxa), and Poa (7 taxa).
Karyotype analysıs of nannospalax xanthodon (spalacıdae, rodentıa) at the easternmost part of ıts dıstrıbutıon range
(Ankara Üniversitesi Fen Fakültesi, 2020) Matur, Ferhat; Other; Other
Chromosomal differentiation can play a crucial role in speciation. In order to reveal a complete picture of the speciation process within a taxon, it is important to determine the geographic distribution of all its chromosomal forms. The blind mole rats (subfamily Spalacinae) are one of the best models for studying chromosomal speciation due to extremely rich chromosomal variation across their large geographic distribution range. To fill the gap in our knowledge of the distribution of chromosomal races of small-bodied blind mole rats (Nannospalax sp.), we collected individuals from the region of Javakheti, Georgia – one of the easternmost localities known for N. xanthodon. We determined that this population has 9 meta- or submetacentric and 15 acrocentric chromosomes (2n=50, NFa=66, NF= 70). The same chromosomal formula is known for the blind mole rats from the nearby Erzurum-Kars plateau in Turkey. We compare our results with the other chromosomal races with the same diploid number 2n=50 found in Anatolia and Eastern Europe.
Nematode-destroyıng fungı: ınfectıon structures, ınteractıon mechanısms and bıocontrol
(Ankara Üniversitesi Fen Fakültesi, 2020) Karakaş, Mehmet; Biyoloji; Fen Fakültesi
Fungi are pathogenic for different nematode groups, but their relationship with soil nematodes goes a grade beyond parasitism and into predation. Approximately, 200 species of taxonomically various fungi can attack active nematodes, which are effective animals nearly 0.1 to 1.0 mm long. Among these nematode-destroying fungi, only a few species are obligate parasites of nematodes; the majority are facultative saprotrophs. Nematode-destroying fungi have four general groups: (a) fungi with specialized structures (b) fungi with toxins; (c) fungi with spore germination; (d) fungi with colony-forming. Nematode-destroying fungi are natural enemies of nematodes in soil ecosystems and have potential as biocontrol agents against plant- and animal-parasitic nematodes. These predator fungi catches free-living nematodes in the soil ecosystem using traps produced by the fungal mycelium that cling to the worm, then, penetrate, kill, and digest the tissue of the nematode. Five kinds of trapping apparatus belonging to fungi are defined. These are adhesive or sticky column, adhesive or sticky knob, adhesive or sticky system, constricting and non-constricting rings.
Reassessment of ıucn threat category for local endemıc campanula damboldtıana from Ankara, Turkey
(Ankara Üniversitesi Fen Fakültesi, 2020) Yaprak, Ahmet Emre; Biyoloji; Fen Fakültesi
In this study, threat category of local endemic plant species Campanula damboldtiana was reassessed according to IUCN Red List Categories and Criteria. C. damboldtiana prefers special habitat type with marly-gypsaceous soils. The assessment based on new field data such as current population size, distribution areas and the main threats to this taxon. Field studies were carried out during the vegetation periods between 2017 and 2018. In addition to those parameters, soil samples were taken from each location and physical and chemical analyses were performed including pH, EC, gypsum, texture, CaCO3 parameters. Also, bioclimatic interpretations were made with the climatic data of the locations. With a recently discovered population, C. damboldtiana has three populations known from Ankara. The estimated total number of mature individuals was 8982. The area of occupancy and the extent of occurrence were calculated as 16 km2 and 16 km2, respectively. Considering our findings of C. damboldtiana, the IUCN threat category was reassessed as Critically Endangered (CR) as indicated in Red Data Book of Turkish Plants.

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