Nadir ve dar yayılışlı Muscari adilii M.B. Güner & H. Duman, Verbascum gypsicola Vural & Aydoğdu ve Kalidium wagenitzii (Aellen) Freitag & G. Kadereit endemik bitki türlerinin ISSR tekniği ile genetik çeşitliliğinin koruma amaçlı belirlenmesi
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Date
2020
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Fen Bilimleri Enstitüsü
Abstract
In this study, the population dynamics and genetic diversity of three Central Anatolian endemic species, Kalidium wagenitzii, Muscari adilii and Verbascum gypsicola were studied for sustainable conservation purposes. Population dynamics of the species were evaluated according to IUCN Red List Criteria and Categories (2012), and for the evaluation of genetic diversity at species and population level PCR-ISSR technique was used. Physical and chemical analysis of soil samples, taken from the distribution areas of each species, provide information about ecological preferences of the species. Climatic condition of the distribution areas of each species were also evaluated. K. wagenitzii has two main populations. It was determined that Eskil populations (5087 individuals), is composed of four subpopulations, has larger distribution area than Gölyazı population (1371 individuals). It was detected that larger subpopulation (D) has lower heterozygosity and genetic diversity than three small subpopulations (A,B,C) of Eskil population. Lower heterozygosity and genetic diversity indicates that these small subpopulations may get in to genetic bottleneck. For this reason, all the activities result in habitat loss and destruction, that lead isolation should be avoided. It was determined that M. adilii has three populations; Hırkatepe (200 individuals), Çoban Ahmet Çeşmesi (119 individuals) and Nallıhan (5795 individuals). Hırkatepe and Çoban Ahmet Çeşmesi populations are under the threat of road construction and forestation, in these two populations high expected heterozygosity and genetic diversity was determined so there should be precautions to avoid loss of population sizes. Solta Pass (335 individuals), Kösebükü (700 individuals), Beypazarı (335 individuals) and Yeşilköy (185 individuals) comprise the four determined populations of V. gypsicola. It was determined that Beypazarı and Yeşilköy populations have highest expected heterozygosity and genetic diversity. These two populations are getting smaller in size because of expansion of agriculture areas and overgrazing which increase the risk of genetic bottleneck, so there should be some conservation activities done to increase the number of individuals of these populations.
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Genetik çeşitlilik, Endmik, ISSR