Localization of plasminogen activator inhibitor type 1 and 2 in preimplantation mouse development in vitro

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dc.contributor.authorMutluay, Duygu
dc.contributor.authorYamazaki, Yukiko
dc.contributor.authorHokutan, Kanani
dc.contributor.authorRosser, Charles J.
dc.contributor.authorFuruya, Hideki
dc.contributor.departmentVeteriner Fakültesitr_TR
dc.date.accessioned2021-10-07T07:18:56Z
dc.date.available2021-10-07T07:18:56Z
dc.date.issued2019-09-09
dc.description.abstractPlasminogen activator inhibitor type 1 (PAI-1) and type 2 (PAI-2) are the major endogenous inhibitors of fibrinolysis, or thrombolysis, as it is effective in blocking the conversion of plasminogen to plasmin. In mammalian embryos, both PAI-1 and PAI-2 proteins are expressed in the trophoblasts during and after implantation, suggesting their critical roles in implantation and placentation during pregnancy. However, it remains unclear how both proteins localize in the early stage embryos before implantation. In this study, 2 cell stage embryos were flushed from the oviducts and cultured to specified stages in medium at 37 ̊C in a 5% CO2 incubator. Embryos were fixed and double immunostained with anti-PAI-1 and anti- PAI-2 antibody. We determined the critical expression and localization patterns of PAI-1 and PAI-2 proteins in murine preimplantation embryos at 2 cell, 8 cell, morula and blastocyst stages by using confocal laser scanning microscope. We found that PAI-1 and PAI-2 constantly express in the embryos during preimplantation development, and these proteins localize in both the cytoplasm and the nucleus of each blastomere regardless of their developmental stage. Our results suggest that PAI-1 and PAI-2 proteins may play roles in early embryonic development before implantation.tr_TR
dc.identifier.endpage361tr_TR
dc.identifier.issn/e-issn1308-2817
dc.identifier.issue4tr_TR
dc.identifier.startpage357tr_TR
dc.identifier.urihttps://doi.org/10.33988/auvfd.533634tr_TR
dc.identifier.urihttp://hdl.handle.net/20.500.12575/75285
dc.identifier.volume66tr_TR
dc.language.isoentr_TR
dc.publisherAnkara Üniversitesitr_TR
dc.relation.isversionof10.33988/auvfd.533634tr_TR
dc.relation.journalAnkara Üniversitesi Veteriner Fakültesi Dergisitr_TR
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıtr_TR
dc.subjectEmbryo developmenttr_TR
dc.subjectPAI-1tr_TR
dc.subjectPAI-2tr_TR
dc.titleLocalization of plasminogen activator inhibitor type 1 and 2 in preimplantation mouse development in vitrotr_TR
dc.typeArticletr_TR

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