Determination of antibiotic susceptibility, ESBL genes and pulsed-field gel electrophoresis profiles of extended-spectrum β-lactamase-containing Escherichia coli isolates

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dc.contributor.authorTutun, Hidayet
dc.contributor.authorKaragöz, Alper
dc.contributor.authorAltıntaş, Levent
dc.contributor.authorKoçak, Nadir
dc.contributor.departmentVeteriner Fakültesitr_TR
dc.date.accessioned2021-10-07T07:56:40Z
dc.date.available2021-10-07T07:56:40Z
dc.date.issued2019-09-09
dc.description.abstractThe purpose of this study was to determine the phenotypic antibiotic susceptibility patterns, extended-spectrum β-lactamase (ESBL) genes and genotypic profiles of ESBL-positive Escherichia coli strains isolated from urine samples obtained from outpatients with urinary tract infection in Turkey. A total of 120 E. coli strains during 2017, 2018, and 2019 (40 patients per year) were examined for antibiotic susceptibility patterns by disc diffusion method, for ESBL genes using PCR and sequencing and for molecular typing by pulsed-field gel electrophoresis (PFGE) method. The isolates were evaluated for their sensitivity to 21 different antibiotics. Four different antimicrobial resistance patterns were determined according to antibiotic susceptibility status of the isolates. The β-lactamase genes detected in the isolates were CTX-M-15 + OXA-1 (n= 14), CTX-M-15 (n= 24), TEM-1 + CTX-M-15 (n= 52), TEM-1 + SHV-12 (n=6), SHV-12 1 (n= 6), TEM-1 + CTX-M-1 (n= 6), TEM-1 + CTX-M-16 (n= 6) and TEM-1 + CTX-M-9 (n= 6). The CTX-M-15 was the most prevalent ESBL enzyme in the isolates. As a result of PFGE analysis performed by XbaI enzyme restriction process, one major PFGE profile and three main groups (Group I-II-III) were observed. While antibiotic resistance profiles of the strains showed four groups (RI-RII-RIII-RIV), PFGE band profiles showed a major group (90% similarity ratio). High ESBL production and decreased susceptibility to broad-spectrum cephalosporins were observed in E. coli strains. In addition, PFGE analysis showed high clonal similarity among E. coli isolates.tr_TR
dc.identifier.endpage416tr_TR
dc.identifier.issn/e-issn1308-2817
dc.identifier.issue4tr_TR
dc.identifier.startpage407tr_TR
dc.identifier.urihttps://doi.org/10.33988/auvfd.571632tr_TR
dc.identifier.urihttp://hdl.handle.net/20.500.12575/75292
dc.identifier.volume66tr_TR
dc.language.isoentr_TR
dc.publisherAnkara Üniversitesitr_TR
dc.relation.isversionof10.33988/auvfd.571632tr_TR
dc.relation.journalAnkara Üniversitesi Veteriner Fakültesi Dergisitr_TR
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıtr_TR
dc.subjectAntimicrobial drug resistancetr_TR
dc.subjectESBL genestr_TR
dc.subjectEscherichia colitr_TR
dc.titleDetermination of antibiotic susceptibility, ESBL genes and pulsed-field gel electrophoresis profiles of extended-spectrum β-lactamase-containing Escherichia coli isolatestr_TR
dc.typeArticletr_TR

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