Evaluation of melamine and cyanuric acid cytotoxicity: an in vitro study on L929 fibroblasts and CHO cell line

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dc.contributor.authorMelekoğlu, Abdullah
dc.contributor.authorEkici, Hüsamettin
dc.contributor.authorArat, Esra
dc.contributor.authorKarahan, Siyami
dc.contributor.departmentVeteriner Fakültesitr_TR
dc.date.accessioned2021-10-08T13:02:43Z
dc.date.available2021-10-08T13:02:43Z
dc.date.issued2020-09-01
dc.description.abstractMelamine and its metabolites pose health concern as they are used in various industrial products including feed and drugs. There are a limited number of studies on melamine and cyanuric acid cytotoxicity and cellular damage without a certain conclusion. The present study aimed to evaluate melamine, cyanuric acid and its combined cytotoxic effects using 3-(4.5-dimethylthiazol-2-yl) methyl thiazole tetrazolium (MTT) bromide test. The study also evaluated apoptotic and necrotic effect using a double staining method of Hoechst 33342 and propidium iodide. Melamine, cyanuric acid and their combination (1:1) were applied to L929 fibroblasts and Chinese hamster ovary (CHO) cells at various concentrations (1000 µg/mL, 500 µg/mL, 250 µg/mL, 125 µg/mL and 62.5 µg/mL). At the highest concentration (1000 µg/mL), the cell viability dropped down approximately to 50% both in CHO cells and L929 cells. Melamine, cyanuric acid and their mixture caused cytotoxicity in CHO cells and L929 fibroblasts in dose-dependent manner. Cell death occurred through both apoptosis and mainly necrosis. Both cell types were more sensitive to the mixture of melamine and cyanuric acid and, furthermore, CHO cells were more sensitive than L929 fibroblasts. As a result, melamine, cyanuric acid and their combination caused cytotoxicity in CHO cells and L929 fibroblasts. Further studies should be conducted in different cell lines. These studies should also aim to reveal the mechanism of cytotoxicity and related pathways.tr_TR
dc.identifier.endpage406tr_TR
dc.identifier.issn/e-issn1308-2817
dc.identifier.issue4tr_TR
dc.identifier.startpage399tr_TR
dc.identifier.urihttps://doi.org/10.33988/auvfd.664059tr_TR
dc.identifier.urihttp://hdl.handle.net/20.500.12575/75354
dc.identifier.volume67tr_TR
dc.language.isoentr_TR
dc.publisherAnkara Üniversitesitr_TR
dc.relation.isversionof10.33988/auvfd.664059tr_TR
dc.relation.journalAnkara Üniversitesi Veteriner Fakültesi Dergisitr_TR
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıtr_TR
dc.subjectApoptosistr_TR
dc.subjectCyanuric acidtr_TR
dc.subjectCytotoxicitytr_TR
dc.titleEvaluation of melamine and cyanuric acid cytotoxicity: an in vitro study on L929 fibroblasts and CHO cell linetr_TR
dc.typeArticletr_TR

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