Browsing by Author "Altuner, Ergin Murat"

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    Bazı karayosunu türlerinin antimikrobiyal aktivitesinin belirlenmesi
    (Fen Bilimleri Enstitüsü, 2008) Altuner, Ergin Murat; Çetin, Barbaros
    Bu çalışmada 20 farklı karayosunun 10 farklı mikroorganizma üzerine antimikrobiyal etkisi in vitro incelenmiştir. Karayosunlarından ekstraksiyon için kloroform, benzen, dietil eter, etil alkol, metil alkol, etil asetat, sdH2O ve 0.5 M Tris - HCl tamponu (pH= 8.0) çözücü olarak kullanılmıştır. Genel olarak, sdH2O ve tampon ekstraktları ile en yüksek, buna karşılık dietil eter ve etil alkol ekstraktları ise en düşük seviyede antimikrobiyal etki göstermiştir. Ayrıca, iki ayrı lokasyondan toplanan 3 karayosunu örneği kullanılarak, lokasyonun antimikrobiyal aktivite üzerine olası etkisi araştırılmış ve lokasyon parametresinin, özellikle de karayosunlarının yaşadığı fiziksel çevrenin antimikrobiyal aktivite açısından etkili olduğu gözlenmiştir. Çalışmada kullanılan karayosunlarından, Isothecium alopecuroides (Dubois) Isov., etki ettiği mikroorganizma sayısı açısından en yüksek etkiyi göstermiştir. Calliergonella cuspidata (Hedw.) Locske, Pterigynandrum filiforme Hedw., Plagiothecium denticulatum (Hedw.) Br. Eur. var. denticulatum ve Pohlia cruda (hedw.) Lindb. (Webera cruda (Hedw.) Bruch. ise test edilen hiçbir mikroorganizmaya etki göstermemiştir. Ayrıca, seçilen karayosunlarından hiçbirinin Salmonella enteretidis ATCC 13076 üzerine antimikrobiyal aktivite göstermediği gözlenmiştir. Sonuçlar; standart cephalothin (30µg), gentamicin (10µg), cefuroxime (30µg), ampicilline (10µg), sulfamethoxazole - trimethoprim (23,75 - 1,25 µg) ve vancomicin (30µg) diskleriyle karşılaştırılarak yorumlanmıştır.AbstractIn this study the antimicrobial activity of 20 different mosses were tested in vitro against 10 different microorganisms. For the extraction, chloroform, benzene, diethyl ether, ethyl alcohol, methyl alcohol, ethyl acetate, sdH2O and 0.5M Tris-HCl buffer (pH=8.0) were used as solvents. According to the results, it could be proposed that among the solvents, solving the active substances the most effective ones are sdH2O and the buffer solution, and the least are diethyl ether and ethyl alcohol. In addition to these, the effect of the location on the antimicrobial activity of the mosses was put forward by using 3 couples of moss samples which were collected from two different locations. As a result, it was observed that the location parameter, especially the physical environment of the mosses affects the antimicrobial activity. Isothecium alopecuroides (Dubois) Isov., one of the moss used in the study, showed the highest activity in terms of the number of microorganism affected. On the other hand, Calliergonella cuspidata (Hedw.) Locske, Pterigynandrum filiforme Hedw., Plagiothecium denticulatum (Hedw.) Br. Eur. var. denticulatum and Pohlia cruda (hedw.) Lindb. (Webera cruda (Hedw.) Bruch. had no antimicrobial effect on any microorganisms tested. Also neither of the mosses tested showed antimicrobial activity against Salmonella enteretidis ATCC 13076. All the results were compared with a series of standard antibiotic discs, cephalothin (30µg), gentamicin (10µg), cefuroxime (30µg), ampicilline (10µg), sulfamethoxazole - trimethoprim (23,75 - 1,25 µg) and vancomicin (30µg).
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    IN SILICO PROOFS FOR PHLORIDZIN, NARINGENIN, AND CINNAMIC ACID AS ALPHA-AMYLASE ACTIVATORS, WHICH IS IMPORTANT IN INDUSTRIAL MICROBIOLOGY OR BIOCHEMICAL ENGINEERING
    (Ankara Üniversitesi Fen Fakültesi, 2021) Altuner, Ergin Murat; Other; Other
    Enzymes are commonly defined as biological catalysts, regulating particular biochemical reactions. α-Amylase (EC 3.2.1.1) is one of the industrially important enzymes, which are extensively used in starch hydrolyzing processes, such as brewing, fermentation, detergent production, food processing, etc. This enzyme breaks down α-1,4 glycosidic bonds in amylose or amylopectin. The end products from amylose are maltotriose and maltose. Maltose, glucose, and limit dextrin are formed from amylopectin. There are many studies in the literature regarding the α-amylase inhibitors, which have the potentials of being used in diabetes and obesity. However, there is a very limited number of studies in the literature about the activation of this enzyme, which could be harmful to such diseases. This study aims to support the activation activity of phloridzin, naringenin, and cinnamic acid for α-amylase, which was previously proved experimentally, with some in silico tests.