Cilt:31 Sayı:01 (2022)

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https://dspace.ankara.edu.tr/handle/20.500.12575/86635

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Browsing Cilt:31 Sayı:01 (2022) by Author "Biyoloji"

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    Ankara Üniversitesi Fen Fakültesi
    (Ankara Üniversitesi Fen Fakütesi, 2022) Atasagun, Sibel; Biyoloji; Fen Fakültesi
    Lemna minor L. has been traditionally used for a long time for its analgesic, antipyretic, vitamin C supplement, astringent, antipruritic effects. Although there are many heavy metal removals using L. minor, unfortunately, biological activity studies are very limited. In this study, the chemical content and total phenol content, DPPH removal, metal chelation (Fe2+) and β-Carotene-lycopene methods of the L. minor macrophyte we obtained from Turkey were determined by GC-MS. The results of the study showed that our plant contains 25 different essential oils and has a high phenol content. In addition, 72% DPPH removal of L.minor was determined when it had 71% iron chelating ability. As a result of our study, it has been revealed that the L. minor we use is an effective antioxidant. It is thought that its usability in the fields of food and medicine can be investigated with further studies.
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    Antioxidant activities of Turkish extra virgin olive oils
    (Ankara Üniversitesi Fen Fakültesi, 2022) Anar, Okan; Biyoloji; Fen Fakültesi
    Extra virgin olive oil is the highest grade of virgin olive oil derived by cold mechanical extraction without the use of solvents or refining methods. These olive oils are known for their composition in phenolic compounds that have antioxidant properties. This study aims to determine the total phenolic and flavonoid compounds and antioxidant activities of four Turkish extra virgin olive oil samples: Kilis yağlık, İzmir sofralık, Ayvalık, and Tavşan yüreği. The highest sample concentration used for the experiments was 4 mg/ml while 1 mg/mL was used for ABTS radical scavenging assay. The lowest total phenolic and flavonoid content was observed in Tavşan yüreği sample. All extra virgin olive oil samples showed scavenging activity against DPPH and ABTS free radicals. Extra virgin olive oil samples with high phenolic and flavonoid content presented more effective radical scavenging activity with low IC50 values. This study provides information about the phenolic content and antioxidant activities of four important Turkish olive oil samples.
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    Detailed characterization of lectin genes in common bean using bioinformatic tools
    (Ankara Üniversitesi Fen Fakültesi, 2022) Aras, Sümer; Biyoloji; Fen Fakültesi
    Because of differences in molecular structure, biochemical properties, and carbohydrate binding specificity, lectins are considered a complex and heterogeneous group of proteins found in all organisms. Plant lectins are important proteins in terms of their benefits in cancer treatments, biomedical applications, and many medical uses due to their numerous biological roles such as intercellular interactions, defense mechanisms formation, immunomodulation, and anticarcinogenic activity. Despite the discovery of significant amounts of lectin proteins in different plant species, many questions about their potential biological role remain unanswered in P. vulgaris L. In this study, using bioinformatics tools, 52 Pvul-LEC genes were identified in the P. vulgaris genome and these genes were clustered into three subgroups based on phylogenetic analysis. The majority of Pvul-LEC proteins in the same subfamily of phylogenetic tree shared similar motifs and gene structures. Eight pairs of segmental duplications were discovered based on genome wide duplication analysis. Pvul-LEC proteins' three-dimensional structure and functions were also predicted. Simultaneously, gene expression levels of Pvul-LEC genes against drought and salt stress in leaf tissues were evaluated based on publicly available RNAseq data. As a result, it is anticipated that the data obtained in the current study will be beneficial to literature and following studies related to lectin genes.